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Tiling Across Viral Genomes with 300mer Oligonucleotides to Investigate Immune Escape
Learn how 300mer oligos can be used to tile across genomes to interrogate otherwise hidden genetic functionality
Viruses exploit high-dimensional RNA structures to facilitate viral replication and impede host restriction. Here, a novel next-generation sequencing (NGS)-based screening workflow, called Fate-seq, was used to define and characterize high-dimensional RNA structures from the severe acute respiratory syndrome coronavirus (SARSCoV) genome in an unbiased, high-throughput, and comprehensive manner. The Fate-seq workflow leverages 300mer Twist Oligos to simplify library preparation and capture genomic sequences long enough to probe RNA secondary structures.
Covered in this Application Note
Details on Fate-seq, a new method for high-throughput analysis of RNA secondary structure
How 300mer oligos can be used to tile across a genome to interrogate its function
How RNA secondary structures in SARS-CoV and SARS-CoV-2 may contribute to immune escape
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